Sample overview

Project Name	TCGA-READ
GSE ID	GSE205506
GSM ID	GSM6213973
Cell Line	None
Tissue type	colon
Disease Type	tumor
PMID	37172580
Initial gene number	33538
Initial cell number	7307
Post-QC gene number	8278
Post-QC cell number	4353

QC Metrics

Bulk-to-ScRNA-seq Label Transfer

Bulk phenotype-associated subpopulations

Reference-Based Annotation

Differentially expressed genes

Overall Survival : Cluster vs

Overall Survival : Cluster vs ( + )

Clinical Stage : Cluster vs

Tumor Size : Cluster vs

Lymph Node Involvement : Cluster vs

Distant Metastasis : Cluster vs

Cell-Cell communications ( Survival-Associated Subpopulations Across Cell Types )

Cell Population Signaling and Communication

Ligand-Receptor-Mediated Cell-Cell Interactions

Sending Cell Type	Ligand	Receiving Cell Type	Receptor	Score	Pathway Name	Function	Evidence
Epithelial,cells(Nos)_source	TGFA_ligand	Epithelial,cells(Nos)_target	EGFR_receptor	3.977e-05	EGF	Secreted Signaling	KEGG: hsa04012
Epithelial,cells(Nos)_source	TGFA_ligand	Epithelial,cells(Nos)_target	EGFR_ERBB2_receptor	6.197e-05	EGF	Secreted Signaling	KEGG: hsa04012
Epithelial,cells(Nos)_source	MIF_ligand	B-cells(Nos)_target	CD74_CD44_receptor	3.289e-05	MIF	Secreted Signaling	PMID: 29637711; PMID: 26175090
Epithelial,cells(Nos)_source	MIF_ligand	Epithelial,cells(Nos)_target	CD74_CD44_receptor	3.309e-04	MIF	Secreted Signaling	PMID: 29637711; PMID: 26175090
Epithelial,cells(Nos)_source	TNFSF10_ligand	Epithelial,cells(Nos)_target	TNFRSF10A_receptor	2.455e-04	TRAIL	Secreted Signaling	KEGG: hsa04060
Epithelial,cells(Nos)_source	NAMPT_ligand	Epithelial,cells(Nos)_target	INSR_receptor	2.417e-03	VISFATIN	Secreted Signaling	PMID: 28490838
Epithelial,cells(Nos)_source	MDK_ligand	B-cells(Nos)_target	SDC1_receptor	2.214e-05	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Epithelial,cells(Nos)_target	SDC1_receptor	2.458e-04	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Epithelial,cells(Nos)_target	SDC4_receptor	2.693e-04	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	B-cells(Nos)_target	ITGA6_ITGB1_receptor	9.608e-09	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	CD8+,T-cells(Nos)_target	ITGA6_ITGB1_receptor	3.090e-06	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Epithelial,cells(Nos)_target	ITGA6_ITGB1_receptor	8.284e-04	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Monocytes(Nos)_target	ITGA6_ITGB1_receptor	1.258e-08	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	B-cells(Nos)_target	LRP1_receptor	4.743e-08	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	CD8+,T-cells(Nos)_target	LRP1_receptor	1.586e-07	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Epithelial,cells(Nos)_target	LRP1_receptor	1.366e-04	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Monocytes(Nos)_target	LRP1_receptor	7.630e-08	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	B-cells(Nos)_target	NCL_receptor	1.274e-05	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	CD8+,T-cells(Nos)_target	NCL_receptor	8.801e-05	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Epithelial,cells(Nos)_target	NCL_receptor	6.093e-04	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	MDK_ligand	Monocytes(Nos)_target	NCL_receptor	1.952e-05	MK	Secreted Signaling	PMID: 28356350
Epithelial,cells(Nos)_source	PRSS3_ligand	Epithelial,cells(Nos)_target	F2RL1_receptor	1.196e-03	PARs	Secreted Signaling	KEGG: hsa04080
Epithelial,cells(Nos)_source	PRSS3_ligand	Epithelial,cells(Nos)_target	PARD3_receptor	3.001e-05	PARs	Secreted Signaling	KEGG: hsa04080
Epithelial,cells(Nos)_source	GRN_ligand	Epithelial,cells(Nos)_target	SORT1_receptor	8.314e-04	GRN	Secreted Signaling	PMID: 29555433
Monocytes(Nos)_source	GRN_ligand	Epithelial,cells(Nos)_target	SORT1_receptor	5.510e-05	GRN	Secreted Signaling	PMID: 29555433
Epithelial,cells(Nos)_source	LGALS9_ligand	CD8+,T-cells(Nos)_target	PTPRC_receptor	1.769e-03	GALECTIN	Secreted Signaling	PMID: 30120235
Epithelial,cells(Nos)_source	LGALS9_ligand	Monocytes(Nos)_target	PTPRC_receptor	1.381e-04	GALECTIN	Secreted Signaling	PMID: 30120235
Epithelial,cells(Nos)_source	LGALS9_ligand	Epithelial,cells(Nos)_target	CD44_receptor	3.751e-04	GALECTIN	Secreted Signaling	PMID: 25065622
Epithelial,cells(Nos)_source	LAMB1_ligand	B-cells(Nos)_target	ITGA1_ITGB1_receptor	1.122e-08	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	CD8+,T-cells(Nos)_target	ITGA1_ITGB1_receptor	2.141e-05	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	ITGA1_ITGB1_receptor	4.742e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Monocytes(Nos)_target	ITGA1_ITGB1_receptor	1.468e-08	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	B-cells(Nos)_target	ITGA2_ITGB1_receptor	1.122e-08	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	CD8+,T-cells(Nos)_target	ITGA2_ITGB1_receptor	3.587e-06	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	ITGA2_ITGB1_receptor	5.136e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Monocytes(Nos)_target	ITGA2_ITGB1_receptor	1.468e-08	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	ITGA3_ITGB1_receptor	2.874e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	B-cells(Nos)_target	ITGA6_ITGB1_receptor	8.138e-09	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	CD8+,T-cells(Nos)_target	ITGA6_ITGB1_receptor	2.617e-06	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	ITGA6_ITGB1_receptor	7.018e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Monocytes(Nos)_target	ITGA6_ITGB1_receptor	1.065e-08	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	B-cells(Nos)_target	ITGA6_ITGB4_receptor	1.867e-09	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	CD8+,T-cells(Nos)_target	ITGA6_ITGB4_receptor	6.050e-07	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	ITGA6_ITGB4_receptor	5.269e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Monocytes(Nos)_target	ITGA6_ITGB4_receptor	2.444e-09	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	B-cells(Nos)_target	CD44_receptor	1.328e-05	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	CD8+,T-cells(Nos)_target	CD44_receptor	4.186e-07	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	CD44_receptor	1.586e-04	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Monocytes(Nos)_target	CD44_receptor	1.901e-07	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	LAMB1_ligand	Epithelial,cells(Nos)_target	DAG1_receptor	8.168e-05	LAMININ	ECM-Receptor	KEGG: hsa04512
Epithelial,cells(Nos)_source	APP_ligand	B-cells(Nos)_target	CD74_receptor	8.455e-04	APP	Cell-Cell Contact	PMID: 19849849
Epithelial,cells(Nos)_source	APP_ligand	Epithelial,cells(Nos)_target	CD74_receptor	7.184e-03	APP	Cell-Cell Contact	PMID: 19849849
Epithelial,cells(Nos)_source	APP_ligand	Monocytes(Nos)_target	CD74_receptor	2.068e-03	APP	Cell-Cell Contact	PMID: 19849849
Epithelial,cells(Nos)_source	CD46_ligand	Epithelial,cells(Nos)_target	JAG1_receptor	4.510e-04	CD46	Cell-Cell Contact	PMID: 23086448
CD8+,T-cells(Nos)_source	CD99_ligand	CD8+,T-cells(Nos)_target	CD99_receptor	2.184e-04	CD99	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	CD99_ligand	CD8+,T-cells(Nos)_target	CD99_receptor	3.012e-04	CD99	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	CD99_ligand	Epithelial,cells(Nos)_target	CD99_receptor	3.012e-04	CD99	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	CDH1_ligand	Epithelial,cells(Nos)_target	CDH1_receptor	2.504e-04	CDH	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	CDH1_ligand	Epithelial,cells(Nos)_target	ITGA2_ITGB1_receptor	6.921e-04	CDH1	Cell-Cell Contact	PMID: 12392763
Epithelial,cells(Nos)_source	CEACAM1_ligand	Epithelial,cells(Nos)_target	CEACAM5_receptor	5.113e-03	CEACAM	Cell-Cell Contact	PMID: 11590190
Epithelial,cells(Nos)_source	DSC2_ligand	Epithelial,cells(Nos)_target	DSG2_receptor	9.200e-03	DESMOSOME	Cell-Cell Contact	PMID: 27298358
Epithelial,cells(Nos)_source	EFNA1_ligand	Epithelial,cells(Nos)_target	EPHA2_receptor	1.927e-05	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA1_ligand	Epithelial,cells(Nos)_target	EPHA4_receptor	5.030e-05	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA4_ligand	Epithelial,cells(Nos)_target	EPHA2_receptor	5.855e-06	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA4_ligand	Epithelial,cells(Nos)_target	EPHA4_receptor	1.529e-05	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA5_ligand	Epithelial,cells(Nos)_target	EPHA2_receptor	8.189e-06	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA5_ligand	Epithelial,cells(Nos)_target	EPHA4_receptor	2.138e-05	EPHA	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNA5_ligand	Epithelial,cells(Nos)_target	EPHB2_receptor	2.928e-05	EPHA	Cell-Cell Contact	PMID:15107857; PMID: 15114347
Epithelial,cells(Nos)_source	EFNB1_ligand	Epithelial,cells(Nos)_target	EPHA4_receptor	1.033e-04	EPHB	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNB1_ligand	Epithelial,cells(Nos)_target	EPHB2_receptor	1.415e-04	EPHB	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNB2_ligand	Epithelial,cells(Nos)_target	EPHA4_receptor	1.056e-04	EPHB	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	EFNB2_ligand	Epithelial,cells(Nos)_target	EPHB2_receptor	1.446e-04	EPHB	Cell-Cell Contact	PMID: 15114347
Epithelial,cells(Nos)_source	F11R_ligand	Epithelial,cells(Nos)_target	F11R_receptor	4.617e-04	JAM	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	MPZL1_ligand	Epithelial,cells(Nos)_target	MPZL1_receptor	2.683e-04	MPZ	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	NECTIN3_ligand	Epithelial,cells(Nos)_target	NECTIN2_receptor	1.180e-04	NECTIN	Cell-Cell Contact	PMID: 23027581
Epithelial,cells(Nos)_source	JAG1_ligand	Epithelial,cells(Nos)_target	NOTCH1_receptor	7.573e-05	NOTCH	Cell-Cell Contact	PMID: 22353464
Epithelial,cells(Nos)_source	OCLN_ligand	Epithelial,cells(Nos)_target	OCLN_receptor	8.255e-05	OCLN	Cell-Cell Contact	KEGG: hsa04514
Epithelial,cells(Nos)_source	SEMA4D_ligand	Epithelial,cells(Nos)_target	PLXNB1_receptor	4.469e-06	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA4D_ligand	Epithelial,cells(Nos)_target	PLXNB2_receptor	2.136e-05	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA4G_ligand	B-cells(Nos)_target	PLXNB2_receptor	9.495e-10	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA4G_ligand	CD8+,T-cells(Nos)_target	PLXNB2_receptor	3.729e-09	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA4G_ligand	Epithelial,cells(Nos)_target	PLXNB2_receptor	1.523e-04	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA4G_ligand	Monocytes(Nos)_target	PLXNB2_receptor	1.340e-09	SEMA4	Cell-Cell Contact	PMID: 27533782
Epithelial,cells(Nos)_source	SEMA6A_ligand	Epithelial,cells(Nos)_target	PLXNA2_receptor	1.776e-04	SEMA6	Cell-Cell Contact	PMID: 27533782