Sample overview

Project Name	TCGA-HNSC
GSE ID	GSE164690
GSM ID	GSM5017034
Cell Line	None
Tissue type	Tumor
Disease Type	None
PMID	34921143
Initial gene number	33545
Initial cell number	2553
Post-QC gene number	1582
Post-QC cell number	2046

QC Metrics

Bulk-to-ScRNA-seq Label Transfer

Bulk phenotype-associated subpopulations

Reference-Based Annotation

Differentially expressed genes

Overall Survival : Cluster vs

Overall Survival : Cluster vs ( + )

Clinical Stage : Cluster vs

Tumor Size : Cluster vs

Lymph Node Involvement : Cluster vs

Distant Metastasis : Cluster vs

Cell-Cell communications ( Survival-Associated Subpopulations Across Cell Types )

Cell Population Signaling and Communication

Ligand-Receptor-Mediated Cell-Cell Interactions

Sending Cell Type	Ligand	Receiving Cell Type	Receptor	Score	P-value	Pathway Name	Function	Evidence
CD8+,T-cells(Nos)_source	CXCL13_ligand	CD8+,T-cells(Nos)_target	CXCR3_receptor	3.258e-03	0.0	CXCL	Secreted Signaling	KEGG: hsa04060
CD8+,T-cells(Nos)_source	CD99_ligand	CD8+,T-cells(Nos)_target	CD99_receptor	5.644e-02	0.0	CD99	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	CLEC2D_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	4.092e-04	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
CD8+,T-cells(Nos)_source	CLEC2D_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	2.636e-03	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
Macrophages(Nos)_source	CLEC2B_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	1.371e-04	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
B-cells(Nos)_source	CD69_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	4.506e-04	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
CD4+,T-cells(Nos)_source	CD69_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	1.204e-03	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
CD8+,T-cells(Nos)_source	CD69_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	7.337e-03	0.0	CLEC	Cell-Cell Contact	PMID: 24223577
Macrophages(Nos)_source	CD69_ligand	CD4+,T-cells(Nos)_target	KLRB1_receptor	2.264e-04	0.01	CLEC	Cell-Cell Contact	PMID: 24223577
CD4+,T-cells(Nos)_source	LCK_ligand	CD8+,T-cells(Nos)_target	CD8 receptor_receptor	1.297e-03	0.0	LCK	Cell-Cell Contact	PMID: 25427562
CD8+,T-cells(Nos)_source	LCK_ligand	CD8+,T-cells(Nos)_target	CD8 receptor_receptor	9.345e-03	0.0	LCK	Cell-Cell Contact	PMID: 25427562
CD4+,T-cells(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.449e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	2.472e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.370e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.155e-03	0.01	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	2.261e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.366e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.288e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	2.355e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	3.407e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
B-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	2.177e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	4.034e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	2.848e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	4.085e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	7.794e-04	0.03	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	5.560e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8A_receptor	1.288e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
B-cells(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	1.743e-03	0.01	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.732e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	2.674e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-A_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.647e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
B-cells(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	1.737e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.415e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	2.446e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-B_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.642e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
B-cells(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	1.801e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.559e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	2.548e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-C_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.687e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
B-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	2.353e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	4.362e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	3.079e-02	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-E_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	4.417e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD4+,T-cells(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	8.460e-04	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
CD8+,T-cells(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	6.034e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514
Macrophages(Nos)_source	HLA-F_ligand	CD8+,T-cells(Nos)_target	CD8B_receptor	1.397e-03	0.0	MHC-I	Cell-Cell Contact	KEGG: hsa04514